FTIR Spectroscopic Study of Aloe vera barbadensis Mill Buds

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Muhammad S. A. Abbasi
Muhammad Aslam Tahir
Sidra Meer


The objective of this research is to investigate number and types of bioactive compounds present in Aloe Vera buds grown in North East Punjab region of Pakistan by FTIR which is simple, nondestructive, cost effective and user friendly. Aloe vera was cultivated under normal atmospheric conditions. After two years, buds (early stage of flowers) were grown on twigs. The buds were plucked and blended with distilled water in National Juicer machine to have a concentrated blend. The blend was filtered to get clear solution, mixed with ethyl acetate and then solvent extracted. The organic part was isolated and dried at water bath at 60°C. Dried sample was analyzed using FTIR spectroscopic analysis. All activities were performed consecutively to avoid photochemical changes. The characteristic FTIR spectral lines have shown different characteristic peaks that correspond to different functional groups indicating presence of bioactive compounds like substituted cyclic alkanes, alkenes, alcohols, phenols, and aromatics etc. have been investigated.

Aloe vera, Aloe vera buds, FTIR, terpenoids, flavonoids, bioactive compounds.

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How to Cite
Abbasi, M. S. A., Tahir, M. A., & Meer, S. (2020). FTIR Spectroscopic Study of Aloe vera barbadensis Mill Buds. Asian Journal of Chemical Sciences, 7(4), 1-6. https://doi.org/10.9734/ajocs/2020/v7i419026
Original Research Article


Ruiz-Bustos E, et al. Antibacterial and antifungal activities of some Mexican medicinal plants. J. Oral Pathol. Med. Food. 2009;12(6):1398–1402.

Verma S, Singh SP. Current and future status of herbal medicines. Vet. World. 2008;1(11):347.

Eshun K, He Q. Aloe vera: A valuable ingredient for the food, pharmaceutical and cosmetic industries—a review. Crit. Rev. Food Sci. Nutr. 2004;44(2):91–96.

Heinrich M. Tom Reynolds (Ed.), Aloes—The Genus Aloe. Medicinal and Aromatic Plants—Industrial Profiles, vol. 38. CRC Press, Boca Raton, 2004. ISBN 0 415 30672 8 (386 pp., Hardcover, US $119.95, bibliographies for each chapter, some figures and tables, index).” Elsevier; 2005.

Ulbricht C, et al. An evidence-based systematic review of Aloe vera by the natural standard research collaboration. J. Herb. Pharmacother. 2008;7(3–40):279–323.

Hu Y, Xu J, Hu Q. Evaluation of antioxidant potential of Aloe vera (Aloe barbadensis Miller) extracts. J. Agric. Food Chem. 2003;51(26):7788–7791.

Vázquez B, Avila G, Segura D, Escalante B. Antiinflammatory activity of extracts from Aloe vera gel. J. Ethnopharmacol.1996;55(1):69–75.

Shalabi M, Khilo K, Zakaria MM, Elsebaei MG, Abdo W, Awadin W. Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells. Asian Pac. J. Trop. Biomed. 2015;5(5):375–381.

Santos J, Oliveira M, Ibáñez E, Herrero M. Phenolic profile evolution of different ready-to-eat baby-leaf vegetables during storage. J. Oral Pathol. Med A. 2014;1327: 118–131.

Salminen A, Lehtonen M, Suuronen T, Kaarniranta K, Huuskonen J. Terpenoids: Natural inhibitors of NF-κB signaling with anti-inflammatory and anticancer potential. Cell. Mol. Life Sci. 2008;65(19):2979–2999.

Liu HL, Jiang WB, Xie MX. Flavonoids: Recent advances as anticancer drugs. Recent Pat. Anticancer. Drug Discov. 2010;5(2):152–164.

Lu J-J et al. Quinones derived from plant secondary metabolites as anti-cancer agents. Anti-Cancer Agents Med. Chem. (Formerly Curr. Med. Chem. Agents). 2013;13(3):456–463.

Tahir MA, Abbasi MSA. FTIR Spectroscopic Analysis of Mentha spicata L.(Garden Mint). Asian J. Chem. Sci. 2020;1–5.

Atherton P. Aloe vera: Magic or medicine?. Nurs. Stand. (through 2013). 1998; 12(41):49.

Sayed MD. Traditional medicine in health care. J. Ethnopharmacol. 1980;2(1):19–22.

Choi S, Son B, Son Y, Park Y, Lee S, Chung M. The wound‐healing effect of a glycoprotein fraction isolated from aloe vera. Br. J. Dermatol. 2001;145(4):535–545.

Ramamurthy N, Kannan S. Fourier transform infrared spectroscopic analysis of a plant (Calotropis gigantea Linn) from an industrial village, Cuddalore dt, Tamilnadu, India. Rom. J. Biophys. 2007; 17(4):269–276.

Shakib Z, Shahraki N, Razavi BM, Hosseinzadeh H. Aloe vera as an herbal medicine in the treatment of metabolic syndrome: A review. Phyther. Res. 2019; 33(10):2649–2660.

Sudarshan R, Annigeri RG, Sree Vijayabala G. Aloe vera in the treatment for oral submucous fibrosis–A preliminary study. J. oral Pathol. Med. 2012;41(10): 755–761.

Bassan P, et al. FTIR microscopy of biological cells and tissue: Data analysis using resonant Mie scattering (RMieS) EMSC algorithm. Analyst. 2012;137(6): 1370–1377.

Baker MJ et al. Using fourier transform IR spectroscopy to analyze biological materials. Nat. Protoc. 2014;9(8):1771.

Kazarian SG, Chan KLA. ATR-FTIR spectroscopic imaging: Recent advances and applications to biological systems. Analyst. 2013;138(7);1940–1951.