Antifungal Activity of Mimosa pudica, Isolation and NMR Characterization of Bioactive Components

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Okoronkwo Joseph Chukwu
Ahamefula Anselm Ahuchaogu
P. O. Ukaogo
A. I. Obike
John Bull Onyekachi Echeme

Abstract

In this study, the whole plant of Mimosa pudica was extracted using absolute ethanol. The crude ethanolic extract and its isolated triterpenoid glycoside were tested for antifungal activity towards Aspergillus flavus and Trichophyton rubrum using Well Diffusion Method. At concentrations of 25 – 100 mg/mL, the extract possesses antifungal activity (from being partially active to very active)   against Aspergillus flavus and Trichophyton rubrum. The TLC profile of the crude extract indicated many compounds with RF values of 0.41, 0.43, 0.56, 0.68, 0.89, and 0.90. The isolation and purification were established using extensive thin layer and column chromatographic processes by employing various solvents with varied polarity. The results of thin layer and column chromatography gave rise to the isolation of a triterpenoid glycoside with RF value of 0.41. The spectral analyses involving 1H-NMR, 13C-NMR, 13C-DEPT, 135COSY, HMBC, HMQC and infrared were useful in proposing the structure of the compound.

Keywords:
Chromatography, antifungal activity, zone of inhibition, Mimosa pudica, secondary metabolites

Article Details

How to Cite
Joseph Chukwu, O., Anselm Ahuchaogu, A., O. Ukaogo, P., I. Obike, A., & Bull Onyekachi Echeme, J. (2017). Antifungal Activity of Mimosa pudica, Isolation and NMR Characterization of Bioactive Components. Asian Journal of Chemical Sciences, 2(4), 1-5. https://doi.org/10.9734/AJOCS/2017/34744
Section
Original Research Article