Fast Detection of Chloramphenicol in Raw Milk Using a Hairpin Aptamer-templated Silver Nanoclusters
Hengchao Li
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China and National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Bin Hu
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China and National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Yuliang Cheng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China and National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Hang Yu
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China and Qingdao Special Food Research Institute, Qingdao, Shandong Province, 266109, China.
Yunfei Xie
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China and National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Weirong Yao
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China and Qingdao Special Food Research Institute, Qingdao, Shandong Province, 266109, China.
Yahui Guo *
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
He Qian
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, School of Food Science and Technology, Jiangnan University, Wuxi, China.
*Author to whom correspondence should be addressed.
Abstract
In this presented work, a facile and efficient method was established for the detection of chloramphenicol (CAP) based on target-induced structure transformation of aptamer. This aptamer DNA with a hairpin structure can coincidentally serve as a template for the synthesis of bright silver nanoclusters (quantum yield 16.36%). The binding of CAP with aptamer DNA could cause the destruction of the hairpin structure, resulting in the quenching of the fluorescence of silver nanoclusters (AgNCs). It costs less than 10 minutes to complete the assay, and excellent sensitivity was achieved with detection limit of 0.052 nmol/L. The selectivity and recovery experiments also demonstrated satisfactory results of this proposed protocol. The method has potential applicability, and provides a new strategy for the development of label-free sensors based on aptamer and AgNCs.
Keywords: Chloramphenicol, silver nanoclusters, aptamer, fast detection