Spectrophotometric determination of Ciprofloxacin Hydrochloride, Moxifloxacin Hydrochloride and Roxithromycin Hydrochloride in Pure Form
M. S. Mohammed
Department of Chemistry, Faculty of Science, Benha University, Benha 13511, Egypt.
H. H. El-Feky *
Department of Chemistry, Faculty of Science, Benha University, Benha 13511, Egypt.
T. Y. Mohammed
Department of Chemistry, Faculty of Science, Benha University, Benha 13511, Egypt.
S. A. Shama
Department of Chemistry, Faculty of Science, Benha University, Benha 13511, Egypt.
*Author to whom correspondence should be addressed.
Abstract
A straightforward, sensitive, and quick spectrophotometric approach was created and validated for the detection of Ciprofloxacin hydrochloride, Moxifloxacin hydrochloride, and Roxithromycin hydrochloride in pure form. These techniques were based upon the fact that these antibiotics produced dark yellow ion pairs when combined with bromophenol blue. The binary complex in universal buffer solution of the optimum pH values was demonstrated at absorption maxima at 633 nm, 632 nm, and 633 nm for the three drugs, respectively, with bromophenol blue. Various parameters, such as the effect of time and the effect of reagent concentration, were optimized. Beer’s law plots were obeyed in the concentration ranges 2– 8 µg ml-1, 1– 4 µg ml-1, and 1– 6 µg ml-1, for the three drugs, respectively, with bromophenol blue. The detection limits were found to be 1.65, 0.83 and 0.79 μg mL-1, respectively, for Ciprofloxacin hydrochloride, Moxifloxacin hydrochloride, and Roxithromycin hydrochloride with bromophenol blue. The correlation coefficient value suggests that all three systems are linearly consistent.
Keywords: Ciprofloxacin hydrochloride, moxifloxacin hydrochloride and roxithromycin hydrochloride, bromophenol blue, Ion pair complex