Evaluation of Antioxidant Contents and Structural Identification by HPTLC and HPLC-MS/MS of Carotenoids in the BF59XCIP1 Variety of Ipomoea batatas L.
Dominique Saga Kaboré *
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso.
Benjamin Bazié
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso and Ecole Polytechnique de Ouagadougou, Ouagadougou, Burkina Faso.
Mahamadi Ouedraogo
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS/CNRST), 03 BP 7192, Ouagadougou 03, Burkina Faso.
Remy K Bationo
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso and Département Substances Naturelles, CNRST/IRSAT, 03 BP 7047, Ouagadougou 03, Burkina Faso.
Moumouni Koala
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso and Institut de Recherche en Sciences de la Santé (IRSS/CNRST), 03 BP 7192, Ouagadougou 03, Burkina Faso.
Constantin M Dabiré
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso and Laboratoire de Chimie et Energie Renouvelables, Université Nazi BONI, 01 BP 1091 Bobo 01, Burkina Faso.
Koussao Somé
CNRST/INERA, Département Productions Végétales, 03 BP 7047 Ouagadougou 03, Burkina Faso.
Eloi Palé
Laboratoire de Chimie Analytique, Environnementale et Bio-Organique (L.C.A.E. BiO.), Université Joseph KI-ZERBO, UFR/SEA 03 BP 7021, Ouagadougou 03, Burkina Faso.
Pierre Duez
Service de Chimie Thérapeutique et de Pharmacognosie, Université de Mons, 25 Chemin du Champ de Mars, 7000 Mons, Belgique.
*Author to whom correspondence should be addressed.
Abstract
Objective: Orange-fleshed sweet potatoes are an important source of nutrients for consumers thanks to the carotenoids they contain. Carotenoids are bioactive compounds which, thanks to their anti-radical properties, can protect the human body against oxidative stress. Oxidative stress is the cause of many degenerative diseases, such as cancer, blindness, and aging. This study aims to evaluate the antioxidant content of the orange-fleshed variety BF59XCIP1, obtained by crossing varieties adapted to local agroecological conditions, and to identify the main carotenoids in this variety.
Study Design: The work combined cutting, tuber harvesting, and various laboratory analyses. This work was carried out as part of my doctoral thesis.
Methodology: Two types of extracts were prepared. Extracts using an acetone-water-acetic acid solvent system (70:29.5:0.5 v/v/v) for the evaluation of antioxidant content and hexane extracts for structural identification. Antioxidant content was evaluated using the DPPH and FRAP methods. HPTLC-MS and HPLC-MS/MS analyses were used for carotenoid identification.
Results: antioxidant content assessed using the DPPH and FRAP methods was estimated at 0.082 mg ET/g and 0.183 mg ET/g of fresh plant material, respectively. For the structural identification of carotenoids, analysis of the two major spots by HPTLC-MS identified β-cryptoxanthin, zeaxanthin, and β-carotene. HPLC-MS/MS analysis identified capsanthin, echinenone, α-tocopherol, and β-carotene.
Conclusion: The BF59XCIP1 variety of orange-fleshed sweet potato is a source of vitamin A. Therefore, its consumption can compensate for vitamin A deficiency in malnourished children.
Keywords: Carotenoids, antioxidant, HPLC-MS/MS, HPTLC-MS