Integrated Chemical Analysis (GC/MS and ¹³C NMR) of the Essential Oil Extracted from Cleistopholis patens (Benth.) Engl. & Diels in Divo (Côte d’Ivoire): Composition and Bioactive Potential
Souleymane Bamba *
Laboratory of Environmental Sciences and Technologies, Faculty of Environment (UFR Environnement), Jean Lorougnon Guédé University, BP 150 Daloa, Côte d’Ivoire.
Atsain-Allangba Marie Rosine
Laboratory of Bio-Organic Chemistry and Natural Substances, Faculty of Fundamental and Applied Sciences (UFR SFA), Nangui Abrogoua University, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Ahou Gisèle Kouakou-Achié
Laboratory of Environmental Sciences and Technologies, Faculty of Environment (UFR Environnement), Jean Lorougnon Guédé University, BP 150 Daloa, Côte d’Ivoire and Laboratory of Matter Constitution and Reactions, Faculty of Sciences of Structures, Matter and Technology (UFR SSMT), Félix Houphouët-Boigny University, 01 BP V34 Abidjan 01, Côte d’Ivoire.
N’Guetta Henri D’Abosso
Laboratory of Environmental Sciences and Technologies, Faculty of Environment (UFR Environnement), Jean Lorougnon Guédé University, BP 150 Daloa, Côte d’Ivoire.
Kouamé Raphaël Oussou
Laboratory of Environmental Sciences and Technologies, Faculty of Environment (UFR Environnement), Jean Lorougnon Guédé University, BP 150 Daloa, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
The essential oil of Cleistopholis patens from Divo (Côte d’Ivoire) was obtained by steam distillation with a yield of 0.24 ± 0.01%. The main compounds identified by gas chromatography–mass spectrometry (GC/MS) and confirmed by ¹³C NMR were D-limonene (14.40%), β-thujene (12.36%), α-pinene (8.17%), β-pinene (7.56%), γ-terpinene (7.35%), terpinen-4-ol (7.05%), and caryophyllene (8.38%). These constituents exhibited strong antibacterial activity against the tested clinical strains, with antibacterial parameter values ranging from 3.125 to 6.25 mg/mL.
Keywords: Gas chromatography–mass, spectrometry, clinical strains, antibacterial