Phytochemical Composition and Evaluation of Antimicrobial Activity of Blepharis linariifolia (Pers.) Seeds
Alsiddig Osama *
Department of Chemistry, Faculty of Science and Technology, Omdurman Islamic University, Sudan
Sufyan Awadelkarim
Department of Chemistry, Faculty of Science and Technology, Omdurman Islamic University, Sudan
Noha Ali
Department of Chemistry, Faculty of Science and Technology, Omdurman Islamic University, Sudan
Safa Khalid
Department of Chemistry, Faculty of Science and Technology, Omdurman Islamic University, Sudan
Shima Mohammed
Department of Chemistry, Faculty of Science and Technology, Omdurman Islamic University, Sudan
Nosiba Hashim
Department of Chemistry, Faculty of Science and Technology, Omdurman Islamic University, Sudan
*Author to whom correspondence should be addressed.
Abstract
Blepharis linariifolia (Pers.) originates from Sudan and grows widely in Africa. The current study has been designed to evaluate the bioactive compounds and antimicrobial activity of the n-hexane extract of Blepharis linariifolia seeds. The extract was analyzed by GC-MS (Model GCMS-QP2010 Ultra, Shimadzu Co., Japan) which revealed the presence of 2-Pentanol, 2-methyl;3-Pentanol, 3-methyl; 2-Hexanone; Hexanal; Benzaldehyde, 4-fluoro; 4-Methyl-4-(tetrahydropyran-2-yl)oxypentane-2,3-dione; 4-methyl-4-[3',4',5',6'-tetrahydro-2'-H-pyranyl-2'-oxy]-2,3-pentanedione; Hexane, 1,1'-oxybis; 1-Pentanol, 2,2-dimethyl; Butanoic acid, 2-ethyl-2-methyl; 2-Heptene, 5-ethyl-2,4-dimethyl; Undeca-4,8-dione; Acetic acid, 4-acetyl-2-isopropyl-5,5-dimethyltetrahydrofuran-2-yl ester; 1-(3,3-dimethyl-bicyclo[2.2.1]hept-2-yl)pentan-2-one 3-cyano-2-oxa-1-ethoxyadamanane; Ethyl 3-methyl-2-oxobutyrate; Heneicosane 4,8,12,16-Tetramethylheptadecan-4-olide; 1,2-Benzenedicarboxylic acid, diisooctyl ester; Hexatriacontane and Dotriacontane. All compounds were identified from the spectral libraries of National Institute of Standard and Technology and WILEY. FTIR analysis revealed the presence various functional groups related to different type of organic substances including: Organic halogen, Ester, nitro compound, aromatics, amines, Aldehyde, Alkenes, Phenol, Alcohol and silicon compounds. The in vitro antimicrobial assays showed there is non-significant activity of this extract against five microorganism tested for.
Keywords: Blepharis linariifolia, oil, GCMS, FTIR, antimicrobial