Antifungal Activity of Mimosa pudica, Isolation and NMR Characterization of Bioactive Components
Okoronkwo Joseph Chukwu
Department of Chemistry, Michael Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria
Ahamefula Anselm Ahuchaogu *
Department of Industrial Chemistry, Abia State University, Uturu, Nigeria
P. O. Ukaogo
Department of Industrial Chemistry, Abia State University, Uturu, Nigeria
A. I. Obike
Department of Industrial Chemistry, Abia State University, Uturu, Nigeria
John Bull Onyekachi Echeme
Department of Chemistry, Michael Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria
*Author to whom correspondence should be addressed.
Abstract
In this study, the whole plant of Mimosa pudica was extracted using absolute ethanol. The crude ethanolic extract and its isolated triterpenoid glycoside were tested for antifungal activity towards Aspergillus flavus and Trichophyton rubrum using Well Diffusion Method. At concentrations of 25 – 100 mg/mL, the extract possesses antifungal activity (from being partially active to very active) against Aspergillus flavus and Trichophyton rubrum. The TLC profile of the crude extract indicated many compounds with RF values of 0.41, 0.43, 0.56, 0.68, 0.89, and 0.90. The isolation and purification were established using extensive thin layer and column chromatographic processes by employing various solvents with varied polarity. The results of thin layer and column chromatography gave rise to the isolation of a triterpenoid glycoside with RF value of 0.41. The spectral analyses involving 1H-NMR, 13C-NMR, 13C-DEPT, 135COSY, HMBC, HMQC and infrared were useful in proposing the structure of the compound.
Keywords: Chromatography, antifungal activity, zone of inhibition, Mimosa pudica, secondary metabolites