Endothelial Nitric Oxide Synthase (eNOS) Gene Polymorphism (G894T) as a Risk Factor for obesity in the Egyptian Population
Rizk Elbaz
Genetics Unit, Faculty of Medicine, Mansoura University, Egypt.
Lamiaa A. A. Barakat
Department of Chemistry, Faculty of Science, Port Said University, Egypt.
Ahmed Nageb *
Department of Chemistry, Faculty of Science, Port Said University, Egypt.
Hewaydah El Sayed
Department of Zoology, Faculty of Science, Tanat University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Background and Objective: Endothelial nitric oxide synthase gene polymorphism (eNOS) is one of three isoforms that synthesize nitric oxide (NO), that participates in several biological processes have been associated with obesity. This study was undertaken to determine if eNOS gene (G894T) was associated with susceptibility of obesity.
Materials and Methods: The study was carried out on 200 cases divided into 100 obese patient and 100 healthy as control. The mean age cases was (27.02 ± 10.90) they include 79 female and 21 males. All participants were subjected to an estimation of their body mass index (BMI), weight hip ratio (WHR), in addition to random blood sugar (RBS), total cholesterol, triglyceride (TG), and lactate dehydrogenase enzyme (LDH). DNA was amplified using PCR-SSP for detection of relation between polymorphism and endothelial nitric oxide synthase gene in G894T.
Results: All cases showed that there were no significant difference between cases and controls regarding to their chemical lab’s analysis (TG, Cholesterol, LDL and HDL).All cases showed significant frequency of G894T GG, TT and GT (P <0.001) vs. Controls
Conclusion: The polymorphism G894T of eNOS considered as a potential risk factor for obesity.
Keywords: Endothelia nitric oxide, gene, polymorphism, obesity