Health Risks Assessment of Heavy Metal Concentration in Cultured Chanos chanos (Bangus) and Scylla serrata (Mudcrab) in Selected Municipalities in Northern Samar

Jaymar L. Erivera

Department of Physical Science, College of Science, University of Eastern Philippines, Philippines and Graduate Studies, University of Eastern Philippines, Philippines.

Ma. Lourdes C. Alvarez

Department of Physical Science, College of Science, University of Eastern Philippines, Philippines and Graduate Studies, University of Eastern Philippines, Philippines.

Tom Jericho L. Abobo

Marine Biology Program, Department of Biological Sciences, College of Science, University of Eastern Philippines, Philippines.

Kristine Rainiel L. Diaz

Graduate School, University of the Philippines Los Banos, Philippines.

May C. Manla

Graduate School, University of the Philippines Los Banos, Philippines and College of Education, Partido State University, Goa, Philippines.

Karina Milagros C. Lim *

Department of Physical Science, College of Science, University of Eastern Philippines, Philippines and Graduate Studies, University of Eastern Philippines, Philippines and University Research and Development Services, University of Eastern Philippines, Philippines.

*Author to whom correspondence should be addressed.


One of the common problem in fishponds is heavy metal contamination. Though there are some heavy metal elements that are naturally occurring, but due to human activities, their concentration goes beyond what is normal. In this study, health risks analysis using Estimated Daily Intake (EDI), Total Hazard Quotient (THQ), Target Cancer Risk (TCR) were done to assess if the quantities of the heavy metals, such as: Arsenic, Cadmium, Chromium, Lead, and Mercury, impose risks to consumer. Arsenic had the highest concentration among all other heavy metals in crab aligue, having 46.83 mg/kg. The consumption of bangus meat may result in an EDI that is greater than PTDI, especially for Arsenic [15.22731-18.10317 μg kg1 BW d1]. Similarly, consuming crab aligue may also result to a high EDI for Arsenic [2.48197-5.27841μg kg1 BW d1]. THQ was also evaluated as well as the sum of individual heavy metal values which is the Hazard Index (HI) that exceeded to 1 multiple times. In terms of TCR levels, all of the heavy metals exceeded the acceptable limit for cancer risks. Shapiro-Wilk Test had shown non-normal distribution of data for EDI, THQ, and TCR. Spearman’s Correlation Test, meanwhile, suggested that there is a significant relationship between the quantities of heavy metals in bangus meat and crab aligue as well as EDI, THQ, and TCR. In general, based on the health risks assessments (EDI, THQ, and TCR), Arsenic, an established carcinogen, can be the greatest contributor in developing risks and disease, while the varying concentration of Chromium and Cadmium in the samples may also pose risks to consumers. This implies that strict management measures should be implemented to mitigate or lessen the discharge of these heavy metals in the aquatic systems.

Keywords: Heavy metals, health risks, THQ, TCR, EDI, hazard index, aquatic systems, developing risks, food sources, food safety

How to Cite

Erivera , J. L., Alvarez , M. L. C., Abobo , T. J. L., Diaz , K. R. L., Manla , M. C., & Lim , K. M. C. (2024). Health Risks Assessment of Heavy Metal Concentration in Cultured Chanos chanos (Bangus) and Scylla serrata (Mudcrab) in Selected Municipalities in Northern Samar. Asian Journal of Chemical Sciences, 14(2), 113–130.


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Jakimska A, Konieczka P, Skóra K, Namieśnik J. Bioaccumulation of Metals in Tissues of Marine Animals, Part I: The Role and Impact of Heavy Metals on Organisms. Polish Journal of Environmental Studies. 2011;20(5):1117-1125. Available: Animals-Part-I-the-Role-and-Impact,88659,0,2.html

Sarkar T, Alam MM, Parvin N, Fardous Z, Chowdhury AZ, Hossain S, Haque, ME, Biswas N.. Assessment of heavy metals contamination and human health risk in shrimp collected from different farms and rivers at Khulna Satkhira region. Bangladesh. Toxicology Reports. 2016; 3:346–350. Available:

Mattia GD, Bravi MC, Laurenti O, Luca OD, Palmeri A, Sabatucii A, Mendico G, Ghiselli A.. Impairment of cell and plasma redox state in subjects professionally exposed to chromium. American Journal of Industrial Medicine. 2004;46:120-125 Available:

O’Brien TJ, Ceryak S, Patierno SR. Complexities of chromium carcinogenesis: Role of cellular response, repair and recovery mechanisms. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2003;533(1-2):3-36 Available:

Agency for Toxic Substances and Disease Registry. Cadmium Toxicity; 2013 Available:

Baby J, Raj J, Biby E, Sankarganesh P, Jeevitha MV, Ajisha SU, Rajan S. Toxic effect of heavy metals on aquativc environment. International Journal of Biological and Chemical Sciences. 2010;4(4):939-952 Available:

Bañares AC, Alvarez ML. Detection of the presence and concentration of heavy metals in selected rivers in the province of Samar. International Journal of Research-Granthaalayah. 2015;3(9):70-86 Available: e/view/IJRG15_B09_78

Castagnetto J, Hennessy S, Roberts V, Getzoff E, Tainer J, Pique M. MDB: The metalloprotein database and browser at the Scripps Research Institute. Nucleic Acids Research. 2002;30(1):379–382. Available:

Rahman MS, Molla AH, Saha N, Rahman A. Study on heavy metals levels and its risk assessment in some edible fishes from Bangshi River, Savar, Dhaka, Bangladesh. Food Chemistry. 2012;134(4): 1847-1854 Available:

Rahman M, Molla A, Arafat S. Status of pollution around Dhaka export processing zone and its impact on Bangshi River water. Bangladesh. Journal of Nature Science and Sustainable Technology. 2010;4(2):91-110 Available: angladesh

Shamshad BQ, Shahidur RK, Tasrena RC. Studies on toxic elements accumulation in shrimp from fish feed used in Bangladesh. Asian Journal of Food and Agro-Industry. 2009;2(4):440-444.

Available: s_accumulation_in_shrimp_from_fish_feed_used_in_Bangladesh

Arsenic contamination in the freshwater fishponds of Pearl River Delta: bioaccumulation and Health Risk Assessment. Environmental Science Pollution Research International. 2013; 20(7):4484-95. Available:

Deocaris C, Diwa R, Tucio P. Assessment of heavy metal levels in an urban river in the Philippines using an unconstrained ordination- and GIS-based approach: evidence of the return of past pollution after the 2013 Typhoon Haiyan (Yolanda). H2Open Journal. 2022;5(3):412-423. Available:

Department of Natural Resources and Environment, Revised Procedures and Standards for the Management of Hazardous Wastes (Revising DAO 2004-36), Administrative Order No; 2023-22. (December 4, 2013)(Phils.) Available:

Gerhardsson L, Dahlin L, Knebel R, Schütz A. Blood lead concentration after a shotgun accident. Environment Health Perspectives. 2002;110(1):115–117. Available:

Hoque MA, Burgess WG, Shamsudduha M, Ahmed KM. Delineating low- arsenic groundwater environments in the Bengal Aquifer System, Bangladesh. Applied Geochemistry. 2011;26(4):614–623. Available:

Irfan M, Hayat S, Ahmad A, Alyemeni MN. Soil cadmium enrichment: Allocation and plant physiological manifestations. Saudi Journal Biological Sciences. 2013;20(1)1–10 Available:

Williams ES, Priya VL, Karim LR. Bioaccumulation of heavy metals in edible tissue of crab (Scylla serrata) from an estuarine Ramsar site in Kerala, South India. Watershed Ecology and the Environment. 2022;4:59-65 Available:

Erdogrul O, Ateş DA. Determination of cadmium and copper in fish samples from Sir and Menzelet Dam Lake Kahramanmaraş, Turkey. Environmental Monitoring and Assessment. 2006;117(1-3):281–290. Available:

Järup L. Hazards of heavy metal contamination. British Medical Bulletin. 2003;68:167–182 Available:

Khan S, Cao Q, Zheng YM, Huang YZ, Zhu YG. Health risks of heavy metals in contaminated soils and food crops irrigated with wastewater in Beijing, China. Environmental Pollution. 2008;152:686-692 Available:

Markowitz M. Lead poisoning. Pediatrics in Review. 2000;21(10):327–335 Available:

Massachusetts Department of Environmental Protection. Default fish ingestion rates and exposure assumptions for human health risk assessments.; 2008 Available: exposure-assumptions-for-human-healt risk/download#:~:text=The%20recommended%20adult%20ingestion%20rate,and%20published%20peer%20reviewed%20studies

Matschullat J. Arsenic in the geosphere – a review. The Science of Total Environment. 2000;249(1–3):297–312. Available:

Dural M, Göksu MZ, Ozak AA, Derici B. Bioaccumulation of some heavy metals in different tissues of Dicentrarchus labrax L, 1758, Sparus aurata L, 1758 and Mugil cephalus L, 1758 from the Camlik lagoon of the eastern coast of Mediterranean (Turkey). Environmental Monitoring and Assessment. 2006;118(1-3):65–74 Available:

Martin S, Griswold W. Environmental Science and Technology Briefs for Citizens: Human health effects of heavy metals (Issue 15). Center for Hazardous Substance Research; 2009. Available:

Minnesota Department of Health. Heavy metals in fertilizers; 2023. Retrieved December 5, 2023.


Griboff J, Wunderlin DA, Monferran MV. Metals, As and Se determination by inductively coupled plasma-mass spectrometry (ICP-MS) in edible fish collected from three eutrophic reservoirs. Their consumption represents a risk for human health. Microchemical Journal. 2017;130:236–244. Available:

Cabahug MR, Ultra VUJ, Morallos SA, Lanuza NG, Espejon EG. Jr Tan, ZN, Bejar FR. Heavy metal concentrations in mollusks and crustaceans harvested from Eastern Samar’s Taft River in the Philippines and its health risks posed to consumers. Philippine Journal of Science. 2023;152(2):1349-1362 Available:

Tayone JC, Ortiz JC, Tayone WC. Selected mollusks from Pujada Bay, Philippines: Heavy metal health risk assessment and antibacterial activities. Asian Journal Biological and Life Science. 2020;9(2):177-184 Available:

Southeast Asian Fisheries Development Center. Fisheries Country Profile: Philippines. SEAFDEC; 2022.

Available: philippines/

Liu Q, Xu X, Zeng J, Shi X, Liao Y, Du P, Tang Y, Huang W, Chen Q, Shou L. Heavy metal concentrations in commercial marine organisms from Xiangshan Bay, China, and the potential health risks. Marine Pollution Bulletin. 2019;141:215–226. Available:

Banada AN, Andel R. Aging in the Philippines. The Gerontologist. 2018;58(2):212–218 Available:

Antoine JM, Fung LA, Grant CN. Assessment of the potential health risks associated with the aluminum, arsenic, cadmium and lead content in selected fruits and vegetables grown in Jamaica. Toxic Reports. 2017;4:181–187. Available:

Cheng Z, Chen KC, Li KB, Nie XP, Wu SC, Wong CK, Wong, MH. 2013.

Centers for Disease Control and Prevention. 2017. Cadmium Factsheet Available:

Da Silva AO, Martinez CBR. Acute effects of Cadmium on osmoregulation of the freshwater teleost Prochilodus lineatusi: Enzymes activity and plasma ions. Aquatic Toxicology. 2014;156:161-168. Available:

Luo W, Wang D, Xu Z, Guoping L, Chen D, Huang X, Wang Y, Yang S, Zhao L, Huang H, Li Y, Wei W, Long Y, Du Z. Effects of cadmium pollution on the safety of rice and fish in a rice-fish coculture system. Environment International. 2020;143:1-10. Available:

Mannzhi M, Edokpayi JN, Durowoju O, Gumbo J, Odiyo JO. Assessment of selected trace metals in fish feeds, pond water, and edible muscles of Oreochromis mossambicus and the evaluation of human health risk associated with its consumption in Vhembe District of Limpopo Province, South Africa. Toxicology Reports. 2021;8:705-717. Available:

Koleli N, Demir A. Chromite. In MNV Prasad, K Shih (Eds.), Environmental Materials and Waste. Elsevier. 2016;245-263.

Wiriawan A, Takarina ND, Pin TG. Analysis of heavy metals (Pb and Zn) concentration in sediment of Blanakan fish ponds, Subang, West Java. In K. A. Sugeng, D. Triyono, & T. Mart (Eds.), International Symposium on Current Progress in Mathematics and Sciences 2016, ISCPMS 2016: Proceedings of the 2nd International Symposium on Current Progress in Mathematics and Sciences 2016 Article 030105 (AIP Conference Proceedings; Vol. 1862). American Institute of Physics Inc; 2017. Available:

University of Toledo. What are the main sources of Lead? 2023). Retrieved November 6, 202 Available: prevention/sources.html

United States Environmental Protection Agency. Indicators: Sediment mercury; 2023. Retrieved on No Novemner 20, 2023. Available: resource-surveys/indicators-sediment- mercury#:~:text=Mercury%20is%20a%20common%20pollutant,and%20animals%20that%20eat%20fish.

Lagniton L. Filipinos’ Consumption of Seafood Falling in Worrying Trend. MaritimeFairtrade; 2022. Available: falling-worrying-trend/

Aledesanmi OT, Agboola FK, Adeniyi IF. Distribution of heavy metals in surface sediments from streams and their associated fishponds in Osun State, Nigeria. Journal of Health and Pollution. 2016;6(11):34-46 Available:https://doi.oíg/10.5696/2156-9614-6-11.34

Food and Agriculture Organization of the United Nations. Milkfish- natural food and feeding habits: development and morphology of the digestive system; 2024 Available: feeding- habits/en/#:~:text=Milkfish%20fry%2C%20juveniles%2C%20and%20adults,1978%3B%20Blaber%2C%201980)

Gnonsoro UP, Asse YE, Sangare NS, Kouakou YU, Trokourey A. Health risk assessment of heavy metals (Pb, Cd, Hg) in Hydroalcoholic Gels of Abidjan, Côte d’Ivoire. Biological Trace Element Research. 2022;200:2510–2518. Available:

Dorne JL, Kass GE, Bordajandi LR, Amzal B, Bertelsen U, Castoldi AF, Heppner C, Eskola M, Fabiansson S, Ferrari P, Scaravelli E, Dogliotti E, Fuerst P, Boobis AR, Verger P. Human risk assessment of heavy metals: Principles and applications. Metal Ions in Life Sciences. 2011;8:27–60. Available:

Fondriest Environmental, Inc. pH of Water; 2013. Retrieved December 5, 2023 Available: quality/ph ://

Smith AH, Lingas EO, Rahman M. Contamination of drinking-water by arsenic in Bangladesh: a public health emergency. Bull World Health Organ. 2000;78(9):1093–1103 Available:

Toxic Substances and Hazardous and Nuclear Wastes Control Act of 1990, Republic Act No. 6969, (October 26, 1990) (Phil.). Available:

Vermont Department of Environmental Conservation. (nd). Sources of Mercury. The Mercury Education and Reduction Campaign. Retrieved December 11, 202 Available:

Keshavarzi B, Hassanaghaei M, Moore F, Mehr MR, Soltanian S, Lahijanzadeh AR, Sorooshian A. Heavy metal contamination and health risk assessment in three (3) commercial fish species in the Persian Gulf. Marine Pollution Bulletin. 2018;129(1):245-252 Available:

United States Environmental Protection Agency. Guidelines for the health risk assessment of chemical mixtures. Federal Register; 1986. Available:

Papanikolaou NC, Hatzidaki EG, Belivanis S, Tzanakakis GN, Tsatsakis AM. Lead toxicity update. A brief review. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research. 2005;11(10):RA329–RA336. Available:

Perelonia KB, Abendanio C, Raña J, Opinion AG, Villeza J, Cambia F. Heavy metal contamination in water and fishery resources in Manila Bay aquaculture farms. The Philippine Journal of Fisheries. 2017;24(2):74-97. Available:

Reilly C. Pollutants in Food – Metals and Metalloids. In: Szefer P, Nriagu JO, (Eds). Mineral Components in Foods. Boca Raton, FL: Taylor & Francis Group. 2007: 363–388.